PKC zeta antibody (70R-30599)

Rabbit polyclonal PKC zeta antibody

Synonyms Polyclonal PKC zeta antibody, Anti-PKC zeta antibody, EC 2.7.11.13 antibody, KPCZ antibody, PKC-zeta antibody, PKC2 antibody, PRKCZ antibody, Protein kinase C zeta type antibody, kinase PKC-zeta antibody, nPKC-zeta antibody
Specificity PKC zeta antibody detects endogenous levels of total PKC zeta protein
Cross Reactivity Mouse, Rat
Applications ELISA, IF, IHC, WB
Immunogen PKC zeta antibody was raised in Rabbit using a synthesized non-phospho derived from human PKC zeta around the phosphorylation site of threonine 410 (T-S-TF-C) as the immunogen

Images

IHC staining using PKC zeta antibody (70R-30599)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta antibody

Specifications

Host Rabbit
Isotype IgG
Form & Buffer Purified IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-1:500, ELISA: 1:20000

Storage & Safety

Storage Store at -20 deg C in small aliquots. Avoid repeated freeze-thaw cycles

General Information

Biological Significance PKC-zeta has an N-terminal regulatory domain, followed by a hinge region and a C-terminal catalytic domain. Second messengers stimulate PKCs by binding to the regulatory domain, translocating the enzyme from cytosol to membrane, and producing a conformational change that removes auto-inhibition of the PKC catalytic protein kinase activity.

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Images

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IHC staining using PKC zeta antibody (70R-30599)



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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta antibody
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Western blot analysis using PKC zeta antibody (70R-30599)



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Western blot analysis of extracts from 3T3 cells treated with PMA (125ng/ml, 30min) using PKC zeta antibody (Lane 1 and 2)
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Immunofluorescent staining using PKC zeta antibody (70R-30599)



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Immunofluorescence analysis of HeLa cells using PKC zeta antibody

Availability: In stock

Price: €230.67
Size: 100 ug
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