||Polyclonal PODXL antibody specific for human PODXL for use in Western Blot.
PODXL is involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family.
||In normal tissue, PODXL is expressed in the kidney glomerular podocytes, where it plays an important part in maintaining filtration pathways, and in endothelial cells in blood vessels. Tissue and Cell Localization: PODXL is an integral component of the glomerular epithelial cell glycocalyx. PODXL lolocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells. Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells.
|Implications in Disease
||PODXL is overexpressed in several types of cancer, e.g. breast, prostate, and testicular cancer. The PODXL protein was identified as a potentially interesting testicular cancer biomarker, and has later been found to be a prognostic biomarker in both colorectal and urothelial cancer.
||PODXL is a member of the sialomucin protein family. PodxL consists of two isoforms, isoform 1 contains 558 amino acids and isoform 2 consists of 526 amino acids with an approximate molecular weight of 58654 and 55404 D.
||PodxL is N- and O-linked glycosylated and undergoes ubiquitination at Lys526.
||Entrez Gene: 5420 Human, Omim: 602632 Human, SwissProt: O00592 Human, Unigene: 732423 Human