POLR2B antibody (70R-12674)

Affinity purified Rabbit polyclonal POLR2B antibody

Synonyms Polyclonal POLR2B antibody, Anti-POLR2B antibody, hRPB140 antibody, POLRB-2 antibody, POLRB-2, hsRPB2 antibody, DNA-directed RNA polymerase II 140 kDa polypeptide antibody, POLRB 2, RPB2 antibody, POL2RB antibody, POLRB 2 antibody, POLR2B, POLR2B antibody, DNA-directed RNA polymerase II subunit B antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen POLR2B antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 172 of RNA polymerase IIB

Images

Western blot analysis using POLR2B antibody (70R-12674)

Western blot analysis of 30 ug of whole cell lysate (A: BCL-1) using a 5 % SDS PAGE gel and POLR2B antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification POLR2B antibody was purified by antigen-affinity chromatography
Molecular Weight 134 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.65 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using POLR2B antibody (70R-12674)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: BCL-1) using a 5 % SDS PAGE gel and POLR2B antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using POLR2B antibody (70R-12674)</font></div>     | Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using POLR2B antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using POLR2B antibody (70R-12674)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 5 % SDS PAGE gel and POLR2B antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using POLR2B antibody (70R-12674)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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