POLR3A protein (His & Thioredoxin tag) (80-1533)
Purified Recombinant POLR3A protein (His tag) (GST tag)
Overview
Overview
| Synonyms | DNA-directed RNA polymerase III largest subunit protein, DNA-directed RNA polymerase III subunit A protein, DNA-directed RNA polymerase III subunit RPC1 protein, hRPC155 protein, POLR-3A protein, RNA polymerase III 155 kDa subunit protein, RNA polymerase III subunit C1 protein, RNA polymerase III subunit C160 protein, RNA polymerase III subunit RPC155-D protein, RPC1 protein, RPC155 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE, WB |
| Assay Information | Currently being validated in ELISA |
Specifications
| Residues | 891-1090 aa |
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| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Method of Purification | POLR3A protein was purified using proprieatary chromatographic techniques |
| Molecular Weight | 39 kDa |
| Tag/Conjugate | His-GST tag |
| Form & Buffer | Supplied in 50 mM Tris buffer, pH 8.0,600 mM NaCL, 1 mM EDTA and 2 mM DTT |
Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -70 deg c for long term storage. Avoid repeat freeze/thaw cycles |
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General Information
| Product Use | Purified Recombinant POLR3A protein for use in SDS-PAGE and Western Blot. |
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| Biological Significance | POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. POLR3A forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. POLR3A also plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. |
| Expression | POLR3A is expressed primarily in the cortex and the white matter of the brain. |
| Implications in Disease | Defects and mutations in POLR3A have been associated with Leukodystrophy, an autosomal recessive neurodegenerative disorder characterized by childhood onset of progressive motor decline manifest as spasticity, ataxia, tremor, and cerebellar signs, as well as mild cognitive regression. Other features may include hypodontia or oligodontia and hypogonadotropic hypogonadism. There is considerable inter- and intrafamilial variability. |
| Sequence Information | POLR3A is a small fragment (aa 891-1090) of the full length protein (1-1390aa). |
| Post-Translational Modifications | POLR3A undergoes ubiquitination at Lys228, Lys406, Lys1013 and Lys1242. |
| Database Links | Entrez Gene: 11128 Human, Omim: 614258 Human, SwissProt: O14802 Human, Unigene: 436896 Human, Protein ID: AAH41089 |
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