PPAR gamma 2 antibody (20R-PR025)
Rabbit polyclonal PPAR gamma 2 antibody
Overview
Overview
| Synonyms | Polyclonal PPAR gamma 2 antibody, Anti-PPAR gamma 2 antibody |
|---|---|
| Specificity | Human |
| Cross Reactivity | To be determined by end - user |
| Applications | IF, WB |
| Immunogen | PPAR gamma-2 antibody was raised in rabbit using a synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2. |
Images
Western blot analysis using PPAR gamma 2 antibody (20R-PR025)
Western blot analysis of PPAR gamma-2 was performed by loading 25 ug of NIH-3T3 (Lane 1), 3T3-L1 (Lane 2), and Hela cell lysates (Lane 3) and a molecular weight protein ladder onto an SDS polyacrylamide gel. PPAR gamma-2 antibody was used at 1/1000.
Specifications
| Host | Rabbit |
|---|---|
| Isotype | IgG |
| Method of Purification | PPAR Gamma 2 antibody was purified by affinity chromatography. |
| Form & Buffer | Purified IgG supplied in PBS with 1 mg/ml BSA and 0.05% NaN3. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | IF: 1:500,WB: 1:500 |
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Storage & Safety
| Storage | Aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor family. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. |
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