PPCDC protein (His tag) (80R-1510)

Purified recombinant Human PPCDC protein

Synonyms COAC protein, PPC-DC protein, MDS018 protein, FLJ14585, Phosphopantothenoylcysteine decarboxylase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PPCDC protein (His tag) (80R-1510)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-204 amino acids: MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 24.6 kDa (224aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4?-hosphopantothenate to 4'-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4'-phosphopantothenate into 4'-phosphopantetheine. Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PPCDC protein (His tag) (80R-1510) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 100 ug
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