PPIL1 protein (30R-1219)

Purified recombinant Human PPIL1 protein

Synonyms PPIL 1, Cyclophilin related gene 1 protein, PPIL-1, Peptidylprolyl isomerase (cyclophilin)-like 1 CGI124 protein, Peptidylprolyl isomerase (cyclophilin) like 1 protein, CYPL1 protein, PPIL-1 protein, rotamase0., PPIL1, PPIL 1 protein, PPIase protein, PPIL1 protein, peptidylprolyl isomerase like 1 protein, Peptidylprolyl isomerase (cyclophilin)-like 1 protein, CGI-124 protein, MGC678 protein, hCyPX protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant PPIL1 protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes

Images

Coomassie Blue stained SDS-PAGE of PPIL1 protein (30R-1219)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-166 amino acids: MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 19.3 kDa (174 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl, pH 8.0, containing 20% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 155 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Peptidylprolyl isomerase (cyclophilin)-like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy. Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of PPIL1 protein (30R-1219) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €169.35
Size: 100 ug
OR
Shipping
View Our Distributors