PPM1G antibody (10R-1101)

Mouse monoclonal PPM1G antibody

Synonyms Monoclonal PPM1G antibody, Anti-PPM1G antibody, Protein phosphatase 1G 'PP2C antibody, MGC1675 antibody, Protein phosphatase 1G antibody, Protein phosphatase 2 catalytic subunit gamma isoform antibody, PPM1G antibody, PPMG-1 antibody, PPM1G, Protein phosphatase 2C gamma isoform antibody, Protein phosphatase magnesium dependent 1 gamma. antibody, PP2Cgamma antibody, MGC2870 antibody, PPMG-1, PPP2CG antibody, PP2C gamma antibody, PP2CG antibody, PPMG 1 antibody, PPMG 1, Protein phosphatase 1C antibody, PPM1G antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen PPM1G antibody was raised in mouse using recombinant human PPM1G (317-546aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of PPM1G antibody (10R-1101)

Cell lysates of 293T(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone k1G6
Isotype IgG1 kappa
Source Anti-human PPM1G antibody, clone k1G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPM1G protein.
Method of Purification PPM1G antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied in liquid form in PBS, pH 7.4, containing 10% Glycerol, 0.01% Sodium Azide
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.

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Images

  • Western Blot analysis of PPM1G antibody (10R-1101) | Cell lysates of 293T(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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