PPM1G protein (His tag) (80R-1906)

Purified recombinant PPM1G protein

Synonyms PP2CG protein, MGC1675 protein, PP2CGAMMA protein, MGC2870 protein, PPMG-1 protein, PPMG 1, PPP2CG protein, PPMG-1, PPMG 1 protein, PPM1G
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PPM1G protein (His tag) (80R-1906)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-546 amino acids: MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 61.4 kDa (566aa)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nM of p-nitrophenyl phosphate (pNPP) per minute at 25 DEG C, pH 7.5 using 10mM of substrate.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that are negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. Recombinant human PPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of PPM1G protein (His tag) (80R-1906) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 50 ug
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