PRMT1 isoform 3 protein (MBP tag) (80R-1042)

Purified recombinant Human PRMT1 isoform 3 protein (MBP tag)

Synonyms Human mRNA for suppressor for yeast mutant complete cds protein, Mrmt 1 protein, HMT 2 protein, IR1B4 protein, PRMT-1, protein arginine methyltransferase 1 protein, HRMT1 L2 protein, Protein arginine N methyltransferase1 protein, HCP1 protein, HMT1 hnRNP methyltransferase protein, Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 protein, HMT1 hnRNP methyltransferase-like 2 isoform 3 protein, HMT1 hnRNP methyltransferase like 2 (S protein, PRMT 1 protein, Interferon receptor 1 bound protein4 protein, PRMT 1 protein, cerevisiae like 2., HRMT1L 2 protein, Mrmt1 protein, PRMT1 protein, Interferon receptor 1 bound protein 4 protein, PRMT 1, Heterogeneous nuclear ribonucleoproteins methyltransferase like2 protein, HMT2 protein, HRMT1L2 protein, PRMT1, IR1 B4 protein, Human mRNA for suppressor for yeast mutant protein, ANM1 protein, HCP 1 protein, Protein arginine N methyltransferase 1 protein, cerevisiae) protein, cerevisiae) like 2 protein, IR1B 4 protein, S protein, HMT1 hnRNP methyltransferase-like 2 isoform 3 ANM 1 protein, PRMT-1 protein, HMT1 (hnRNP methyltransferase S protein, HMT1 hnRNP methyltransferase like 2 protein, Interferon receptor 1bound protein 4 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37 DEG C.

Images

Coomassie Blue stained SDS-PAGE of PRMT1 isoform 3 protein (MBP tag) (80R-1042)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-353 amino acids: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 84 kDa (750 aa)
Tag/Conjugate MBP tag
Form & Buffer Supplied as a liquid in 40mM Tris, pH 8.0, containing 100mM NaCl, 4mM MgCl2, 2mM DTT, and 40% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity 10,000unit/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PRMT1 isoform 3 protein (MBP tag) (80R-1042) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €169.35
Size: 100 ug
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