Profilin 1 protein (30R-1376)
Purified recombinant Human Profilin 1 protein
|Synonyms||Pfn protein, Actin binding protein protein, Profilin -1, PFN1 protein, ProfilinI., PFN 1 protein, Profilin 1 protein, Profilin -1 protein, Profilin 1, Profilin 1, Profilin1 protein, Profilin I protein|
Coomassie Blue stained SDS-PAGE of Profilin 1 protein (30R-1376)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-140 amino acids: MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY|
|Grade & Purity||> 95% pure|
|Molecular Weight||15.0 kDa (140aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Profilin 1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals. Recombinant Profilin1 protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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