Prosurfactant Protein C antibody (20R-PR030)
Rabbit polyclonal Prosurfactant Protein C antibody
Overview
Overview
| Synonyms | Polyclonal Prosurfactant Protein C antibody, Anti-Prosurfactant Protein C antibody, pro-Surfactant Protein C antibody, proSP-C antibody |
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| Cross Reactivity | Human, mouse, rat |
| Applications | ELISA, IHC, WB |
| Immunogen | Prosurfactant protein C antibody was raised in rabbit using recombinant fusion protein containing GST and amino acids.. 1-20 from the n-terminal of the human SP-C proprotein as the immunogen. |
Images
Western blot analysis using Prosurfactant Protein C antibody (20R-PR030)
Western Blot Analysis: Rat lung lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Prosurfactant Protein C (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP
Specifications
| Host | Rabbit |
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| Form & Buffer | Neat rabbit antiserum, with no preservatives. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:50,000, IHC: 1:2,000-1:4,000, WB: 1:5,000 |
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Storage & Safety
| Storage | Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles |
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General Information
| Biological Significance | Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP C is a fragment of a much larger precursor protein of 21 kD. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain. |
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