Proteasome 20S alpha 7 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 10 and 247 of Proteasome 20S alpha 7
Western blot analysis using Proteasome 20S alpha 7 antibody (70R-12603)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Proteasome 20S alpha 7 antibody at a dilution of 1:1000
Proteasome 20S alpha 7 antibody
Western blot analysis using Proteasome 20S alpha 7 antibody (70R-12603)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Proteasome 20S alpha 7 antibody at a dilution of 1:1000
Western blot analysis using Proteasome 20S alpha 7 antibody (70R-12603)
Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299; C: Hela; D: Hep G2) using a 12 % SDS PAGE gel and Proteasome 20S alpha 7 antibody at a dilution of 1:1000
Immunohistochemical staining using Proteasome 20S alpha 7 antibody (70R-12603)
Immunohistochemical staining of paraffin-embedded CL1-5 xenograft using Proteasome 20S alpha 7 antibody at a dilution of 1:100
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Proteasome 20S alpha 7 antibody was purified by antigen-affinity chromatography
Molecular Weight
28 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication.