Protein A antibody (20C-CR7028GP)

Goat polyclonal Protein A antibody

Synonyms Polyclonal Protein A antibody, Anti-Protein A antibody
Specificity Staphylococcus aureus protein A
Cross Reactivity To be determined by end - user
Applications ELISA, IHC, WB
Immunogen Protein A antibody was raised in goat using Protein A from Staphylococcus aureus as the immunogen.

Specifications

Host Goat
Method of Purification Protein A antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form & Buffer Supplied as a liquid in 20 mM Potassium Phosphate and 150 mM Sodium Chloride, pH 7.2, with 0.01% NaN3 added as a preservative.

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain.

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Availability: In stock

Price: €89.38
Size: 1 ml
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