Protein A antibody (rhodamine) (60C-CF0602)
Goat polyclonal Protein A antibody (rhodamine) conjugated
Overview
Overview
| Synonyms | Polyclonal Protein A antibody (rhodamine), Anti-Protein A antibody (rhodamine) |
|---|---|
| Specificity | Staphylococcus aureus protein A |
| Cross Reactivity | To be determined by end-user |
| Applications | IF |
| Immunogen | Protein A antibody (rhodamine) was raised in goat using Protein A [Staphylococcus aureus] as the immunogen. |
Images
Western Blot analysis using Protein A antibody (rhodamine) (60C-CF0602)
Protein A antibody was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Protein A antibody (rhodamine) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | Rhodamine |
| Form & Buffer | IgG fraction supplied as a liquid in 20mM Potassium Phosphate and 150mM NaCl, pH 7.2, with 10mg/ml BSA (IgG and Protease free), with 0.01% NaN3. |
Usage & Assay Information
| Usage Recommendations | IF: 1:500-1:2,500 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. |
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