Protein A protein (30R-1304)
Purified recombinant S.aureus Protein A protein
Overview
Overview
| Synonyms | SPA protein, Immunoglobulin G binding protein A protein, , Immunoglobulin G binding protein A Staphylococcal protein A protein |
|---|---|
| Species | S.aureus |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of Protein A protein (30R-1304)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 37-469 amino acids: MAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DNNKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNKKPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQPA NHADANKAQA LPET |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 48.1 kDa (434aa), confirmed by MALDI-TOF. |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | < 1.0 EU per ug of protein (determined by LAL method) |
General Information
| Biological Significance | Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography. |
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