Heat Shock proteins
Heat shock proteins (HSPs) are present in all cells under normal conditions, but when the cell is exposed to a sudden temperature fluctuation or other stresses such as altered pH or oxygen deprivation, then HSPs are expressed at high levels. HSPs stabilize proteins and are involved in the folding of denatured proteins. High temperatures and other stresses, make it more difficult for proteins to form their proper structures and cause some already structured proteins to unfold. Left uncorrected, misfolded proteins form aggregates that may eventually kill the cell.
Heat shock proteins are of interest to anyone studying cellular biology, as well as an indicator of cellular stress and their role in reducing environmental damage to cells, they have also been implicated as intracellular chaperones, they are heavily involved in cardiovascular biology, HSPs also take part in the innate immune response by binding antigens and presenting them to the immune system.
Fitzgerald Industries supplies a wide range of heat shock proteins for use in ELISA assays, immunohistochemistry, western blotting and more. Our high quality HSP proteins are available from human and E.coli sources as well as conjugated to commonly used tags such as GST, FITC, Histadine, biotin etc.
Some of our most popular heat shock proteins include:
HSP90B1 is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic HSP90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. This HSP90B1 protein is ideal for use in SDS-PAGE applications.
HSP70 is an important part of the cell's machinery for protein folding, and helps to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. This HSP70 protein is >95% pure and conjugated to a Histadine tag.
HSP27 a small heat shock protein, its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. This HSP27 protein is ideal for use in Western blotting applications.