PRPH antibody (70R-10635)
Affinity purified Chicken polyclonal PRPH antibody
Overview
Overview
| Synonyms | Polyclonal PRPH antibody, Anti-PRPH antibody, NEF4 antibody, Peripherin antibody, PRPH1 antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | PRPH antibody was raised in Chicken using full-length recombinant peripherin protein as the immunogen. |
Images
Immunofluorescent staining of PRPH antibody (70R-10635)
Dissociated cell culture prepared from neonatal dorsal root ganglia. Peripherin (green) stains neuronal cell bodies. As controls, rabbit NFL antibodies (red) were used to costain neurites, and DAPI (blue) was used to demonstrate nuclei. Picture courtesy of Dr. Gerry Shaw, University of Florida.
Specifications
| Host | Chicken |
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| Method of Purification | PRPH antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as an antimicrobial agent and 1% BSA added to prevent absorption to theplastic. |
| Concentration | 2 mg/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:2000-1:5000; IHC: 1:2000-1:5000; WB: 1:5000-1:10000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | PRPH is a cytoskeletal protein found in neurons of the peripheral nervous system. PRPH is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin. |
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