Blocking peptides

Blocking peptides are peptides that bind specifically to the target antibody and block undesirable antibody binding. This occurs because the peptide resembles the epitope for which the antibody is specific. Antibodies which become bound to the blocking peptide are no longer able to bind to the epitope in the protein. This mechanism is extremely useful when non-specific binding is an issue, such as immunohistochemistry (IHC) and in Western blotting (immunobloting). Pre-adsorption with blocking peptides can greatly improve experimental results in these procedures.

Fitzgerald Industries supplies a wide range of blocking peptides for thousands of antigen targets. Our range of blocking peptides are the ideal choice for anyone looking more consistent results from their procedures with less signal noise and less false positives.

Some of our most popular blocking peptides include:

SSX2 Blocking Peptide

SSX2 is a transcriptional repressor; it is also increasingly studied as a potentially useful targets in cancer vaccine-based immunotherapy. This SSX2 Blocking Peptide is ideally suited to immunohistochemistry and Western blotting and is specifically matched to this SSX2 antibody.

KLK2 Blocking Peptide

Kallikreins such as KLK2 are a subgroup of serine proteases that are clustered on chromosome 19.  KLK2 is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. This KLK2 Blocking Peptide is perfect for pre-adsorption before immunohistochemistry and/or Western blotting applications with its partner KLK2 antibody.

FZD4 Blocking Peptide

FZD4 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins and this protein may play a role as a positive regulator of this pathway. This FZD4 Blocking Peptide gives very specific blocking activity when used with its matched antibody FZD4 antibody shown below to stain human skin cells.

Immunohistochemical staining of human skin cells using 70R-7474

SPDEF Blocking Peptide

SPDEF belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as a transactivator of prostate-specific antigen. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors. This SPDEF Blocking Peptide is ideally suited to immunohistochemistry and Western blotting, when used with our SPDEF antibody.

MKL1 Blocking Peptide

MKL or megakaryoblastic leukemia or myocardin-like protein 1 acts as a transcriptional coactivator of serum response factor (SRF) and is essential for skeletal myogenic differentiation. This MKL1 Blocking Peptide is excellent for validating specific binding of MKL1 antibody in IHC and WB.

All of our Blocking Peptides can be ordered along with their antibody partner for use in WB and IHC. We also have a comprehensive protocol for using Blocking peptides as Controls in WB and IHC.