PYCR1 protein (His tag) (80R-1572)
Purified recombinant Human PYCR1 protein
|Synonyms||PIG45 protein, PYCR 1, PRO3, PYCR-1, PYCR 1 protein, Pyrroline-5-carboxylate reductase 1 protein, ARCL2B protein, PYCR-1 protein, PYCR1, PP222 protein, P5CR protein, P5C protein|
Coomassie Blue stained SDS-PAGE of PYCR1 protein (His tag) (80R-1572)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-319 amino acids: MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD|
|Grade & Purity||> 90% pure|
|Molecular Weight||35.5 kDa(339aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.5, containing 10% glycerol.|
Usage & Assay Information
|Bioactivity||Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PYCR1 is a universal housekeeping enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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