RAB21 protein (His tag) (80R-1665)
Purified recombinant Human RAB21 protein
|Synonyms||RAB21, RAB-21 protein, RAB-21, RAB 21, , Ras-related protein Rab-21 protein, RAB 21 protein|
Coomassie Blue stained SDS-PAGE of RAB21 protein (His tag) (80R-1665)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||18-222 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMYSF KVVLLGEGCV GKTSLVLRYC ENKFNDKHIT TLQASFLTKK LNIGGKRVNL AIWDTAGQER FHALGPIYYR DSNGAILVYD ITDEDSFQKV KNWVKELRKM LGNEICLCIV GNKIDLEKER HVSIQEAESY AESVGAKHYH TSAKQNKGIE ELFLDLCKRM IETAQVDERA KGNGSSQPGT ARRGVQIIDD EPQAQTSGGG CC|
|Grade & Purity||> 90% pure|
|Molecular Weight||27.1 kDa (242aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT, 200mM NaCl and 1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ras-related protein Rab-21, also known as RAB21, is a member of the Ras superfamily of small GTP-binding proteins. Rab21 is integrin-associated protein and positive regulators of integrin traffic. This protein associate with the cytoplasmic domains of alpha-integrin chains and their expression influences the endo/exocytic traffic of integrins. This function of Rab21 is dependent on its GTP/GDP cycle and proper membrane targeting. Recombinant human RAB21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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