Rabbit anti Bovine IgG (HRP) (43C-CB0134)
Rabbit anti Bovine IgG secondary antibody (HRP)
Overview
Overview
| Synonyms | Rabbit anti Bovine Immunoglobulin G secondary antibody (HRP) |
|---|---|
| Specificity | Bovine IgG |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Rabbit anti-bovine IgG (HRP) was raised in rabbit using bovine IgG F(ab')2 fragment as the immunogen. |
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Rabbit anti Bovine IgG (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | HRP |
| Form & Buffer | Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. |
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