Rabbit anti Goat IgG Fc (rhodamine) (43R-1083)
Rabbit anti Goat IgG Fc fragment secondary antibody (rhodamine)
Overview
Overview
| Synonyms | Rabbit anti Goat Immunoglobulin G Fc secondary antibody (rhodamine) |
|---|---|
| Specificity | This antibody reacts with heavy chains on Goat IgG. |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin Goat serum proteins light chains on all Goat immunoglobulins. |
| Applications | FC, IF, IHC |
| Immunogen | Purified Goat IgG |
Specifications
| Host | Rabbit |
|---|---|
| Source | Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. |
| Grade & Purity | > 95% pure |
| Method of Purification | Affinity purified using solid phase Goat IgG (H&L) |
| Tag/Conjugate | Rhodamine |
| Form & Buffer | Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications |
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Storage & Safety
| Storage | Store at 2-8 deg C. |
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General Information
| Biological Significance | The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system. In IgG, IgA and IgD antibody isotypes, the Fc region is composed of two identical protein fragments, derived from the second and third constant domains of the antibody's two heavy chains; IgM and IgE Fc regions contain three heavy chain constant domains (CH domains 2-4) in each polypeptide chain. |
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