Rabbit anti Goat IgG (H + L) (rhodamine) (43R-1059)
Rabbit anti Goat IgG (H + L) secondary antibody (rhodamine)
Overview
Overview
| Synonyms | Rabbit anti Goat Immunoglobulin G (H + L) secondary antibody (rhodamine) |
|---|---|
| Specificity | This antibody reacts with heavy chains on Goat IgG and light chains on all Goat immunoglobulins. |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin Goat serum proteins. |
| Applications | FC, IF, IHC |
| Immunogen | Purified Goat IgG (H&L) |
Specifications
| Host | Rabbit |
|---|---|
| Source | Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. |
| Grade & Purity | > 95% pure |
| Method of Purification | Affinity purified using solid phase Goat IgG (H&L) |
| Tag/Conjugate | Rhodamine |
| Form & Buffer | Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications |
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Storage & Safety
| Storage | Store at 2-8 deg C. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
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