Rabbit anti Human IgA (40C-CB9114)
Rabbit anti Human IgA secondary antibody
Overview
Overview
| Synonyms | Rabbit anti Human Immunoglobulin A secondary antibody |
|---|---|
| Specificity | Human IgA |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Rabbit anti-human IgA was raised in rabbit using human IgA alpha heavy chain as the immunogen. |
Images
Western Blot analysis using Rabbit anti Human IgA (40C-CB9114)
Lane 1: Human IgA. Load: 100 ng per lane. Primary antibody: Human IgA (alpha chain) antibody at 1:1000 for 60 min at RT. Secondary antibody: DyLight 649 Goat anti-Rabbit at 1:20, 000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT.
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Rabbit anti Human IgA was purified by affinity chromatography. |
| Form & Buffer | 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:5,000-1:20,000, IHC: 1:1,000-1:5,000, WB: 1:500-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. More IgA is produced in mucosal linings than all other types of antibody combined; between three and five grams are secreted into the intestinal lumen each day. This accumulates to 75% of the total immunoglobulin produced in the entire body. |
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