Rabbit anti Mouse IgG2a (Texas Red) (43C-CB5125)
Rabbit anti Mouse IgG2a secondary antibody
Overview
Overview
| Synonyms | Rabbit anti Mouse Immunoglobulin G2a secondary antibody |
|---|---|
| Specificity | Murine IgG2a |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, FC, IF |
| Immunogen | Rabbit anti-mouse IgG2a was raised in rabbit using murine IgG2a heavy chain as the immunogen. |
Images
Western blot analysis using Rabbit anti Mouse IgG2a (Texas Red) (43C-CB5125)
Western blot of Texas Red conjugated Mouse IgG2a (Gamma 2a chain) secondary antibody. Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. None. Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain)..
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Rabbit anti Mouse IgG2a was purified by affinity chromatography. |
| Tag/Conjugate | Texas Red |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). |
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