Rabbit anti Rat IgG (H + L) (Fab'2) (41C-CJ0307)
Rabbit anti Rat IgG (H + L) secondary antibody (Fab'2)
Overview
Overview
| Synonyms | Rabbit anti Rat Immunoglobulin G (H + L) secondary antibody (Fab'2) |
|---|---|
| Specificity | Rat IgG (H + L) |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC |
| Immunogen | Rabbit anti-rat IgG (H+L) (Fab'2) was raised in rabbit using rat IgG whole molecule as the immunogen. |
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Rabbit anti Rat IgG (H + L) (Fab'2) was purified by affinity chromatography. |
| Form & Buffer | 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:60,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody. |
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