Rabbit IgG (HRP) (65C-CH1103)
Purified Rabbit IgG HRP conjugate
|Synonyms||Rabbit Immunoglobulin G (HRP)|
SDS-PAGE analysis of Rabbit IgG (HRP) (65C-CH1103)
Lane M: 3 uL MW Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Peroxidase Conjugated. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
|Method of Purification||Rabbit IgG (HRP) was purified by affinity chromatography.|
|Form & Buffer||Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin sulfate. DO NOT add naN3. Immunoglobulin and protease free.|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.|
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