Rad54 antibody (70R-12568)

Affinity purified Rabbit polyclonal Rad54 antibody

Synonyms Polyclonal Rad54 antibody, Anti-Rad54 antibody, transcriptional regulator ATRX antibody, Rad 54 antibody, alpha thalassemia/mental retardation syndrome X-linked antibody, RAD54L antibody, Rad 54, ATR2 antibody, SHS antibody, RAD54 homolog antibody, X-linked helicase II antibody, X-linked nuclear protein antibody, ATP-dependent helicase ATRX antibody, Zinc finger helicase antibody, RAD54 antibody, alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog S. cerevisiae) antibody, helicase 2 X-linked antibody, ATRX antibody, ZNF-HX antibody, MGC2094 antibody, SFM1 antibody, DNA dependent ATPase and helicase antibody, MRXHF1 antibody, XH2 antibody, Rad-54 antibody, Rad-54, XNP antibody, Rad54
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen Rad54 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 2161 and 2443 of Rad54

Images

Western blot analysis using Rad54 antibody (70R-12568)

Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 5 % SDS PAGE gel and Rad54 antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification Rad54 antibody was purified by antigen-affinity chromatography
Molecular Weight 283 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.6 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using Rad54 antibody (70R-12568)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 5 % SDS PAGE gel and Rad54 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using Rad54 antibody (70R-12568)</font></div>     | Immunohistochemical staining of paraffin-embedded A549 xenograft using Rad54 antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using Rad54 antibody (70R-12568)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:293T; B:Hep G2; C:Raji) using a 5 % SDS PAGE gel and Rad54 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Immunofluorescent staining using Rad54 antibody (70R-12568)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATRX antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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