RAG2 antibody (70R-13300)

Affinity purified Rabbit polyclonal RAG2 antibody

Synonyms Polyclonal RAG2 antibody, Anti-RAG2 antibody, RAG-2, RAG2 antibody, RAG 2, RAG-2 antibody, RAG 2 antibody, RAG2, recombination activating gene 2 antibody, V(D)J recombination-activating protein 2 antibody, RAG-2 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB

Images

Immunofluorescent staining using RAG2 antibody (70R-13300)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification RAG2 antibody was purified by antigen-affinity chromatography
Molecular Weight 59 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.37 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein that is involved in the initiation of VJ recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using RAG2 antibody (70R-13300)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using RAG2 antibody (70R-13300)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Molt-4) using a 7.5 % SDS PAGE gel and RAG2 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using RAG2 antibody (70R-13300)</font></div>     | Immunohistochemical staining of paraffin-embedded DLD1 Xenograft using RAG2 antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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