RANK protein (30R-AS060)

Purified recombinant Human RANK protein

Synonyms OPGL protein, TRANCE protein, TNF-related activation-induced cytokine protein, ODF protein, Osteoclast differentiation factor protein, TNFSF11 protein, soluble Receptor Activator of NFkB Ligand protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of RANK protein corresponding to amino acids PAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID.
Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 19.4 kDa
Form & Buffer Supplied as a lyophilized powder from 5 mM sodium phosphate pH 7.6, 75 mM NaCl.

Usage & Assay Information

Bioactivity Determined by its ability to induce NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG.

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Availability: In stock

Price: €211.69
Size: 10 ug
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