RANKL protein (Mouse) (30R-2217)
Purified recombinant Mouse RANKL protein
Overview
Overview
| Synonyms | Osteoclast Differentiation Factor protein, TRANCE protein, Osteoprotegerin ligand protein, ODF protein, RANKL protein, Receptor Activator of Nuclear Factor kappa B Ligand protein, OPGL protein |
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| Species | Mouse |
| Protein Type | Recombinant |
| Applications | ELISA, Functional Assay |
| Assay Information | The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5 pg/ml. This corresponds to a specific activity of greater than or equal to 2 x 10e8 Units/mg. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 90% pure |
| Method of Purification | RANKL protein was purified by affinity chromatography. |
| Molecular Weight | 20 kDa |
| Form & Buffer | Supplied as a sterile liquid with phosphate buffered saline, pH7.2, 1.0% BSA. 0.22 um filtered. |
Usage & Assay Information
| Bioactivity | The ED50, as measured by induction ofosteoclast formation on mouse RAW264.7 cells, is 4.8ng/ml. |
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Storage & Safety
| Storage | Store at -70 deg C or less. |
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| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | RANK Ligand, also known as TNF-related activation-induced cytokine, along with RANK are members of the TNF superfamily. RANK-L can be found as a soluble protein expressed by activated T lymphocytes, which plays a role in dendritic cell maturation. In addition it has also been shown to be cleaved by TACE releasing an ectodomain variant of the cell bound form. Lastly RANK-L is expressed as a Type II transmembrane protein expressed by osteoblast lineages. The receptor RANK, located on osteoclastic and dendritic cells can recognize all forms of RANK-L. RANK-L plays a critical role in bone development and mineralization. High levels of RANK-L have been associated with degenerative bone diseases. |
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