RAP1A protein (His tag) (80R-1361)

Purified recombinant Human RAP1A protein

Synonyms RAPA-1, Ras related protein Rap 1A protein, KREV 1 protein, RAPA 1, C21KG protein, SMGP21, Ras-related protein Rap-1A protein, RAP 1A protein, G 22K protein, Ras related protein Krev 1 protein, RAP1A member of RAS oncogene family protein, OTTHUMP00000013741 protein, RAPA 1 protein, GTP binding protein smg p21A protein, RAS related protein RAP1A protein, RAP1A, KREV1 protein, G-22K protein, RAP1 protein, RAPA-1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of RAP1A protein (His tag) (80R-1361)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-181 amino acids: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 22.8 kDa (201aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 40% glycerol, 5mM DTT, 200mM NaCl
Concentration 0.25 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides is in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of RAP1A protein (His tag) (80R-1361) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 50 ug
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