Recoverin protein (30R-1063)
Purified recombinant Human Recoverin protein
|Synonyms||Protein CAR protein, Cancer associated retinopathy protein protein, Cancer associated retinopathy protein antibody protein, RCVRN protein, , Recoverin protein, CAR protein protein, RCV1 protein, Cancer-associated retinopathy protein protein, Recoverin protein|
Coomassie Blue stained SDS-PAGE of Recoverin protein (30R-1063)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA|
|Grade & Purity||> 95% pure|
|Molecular Weight||23 kDa (200 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 8.0, containing 1mM EDTA, 2mM MgCl2, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Recoverin is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome. Recombinant human recoverin was overexpressed in E. coli and purified by using conventional chromatography techniques|
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