Retinoblastoma antibody (Thr826) (70R-33655)

Rabbit polyclonal Retinoblastoma antibody (Thr826)

Synonyms Polyclonal Retinoblastoma antibody, Anti-Retinoblastoma antibody, RB1 antibody, retinoblastoma 1 antibody, BOS_12359 antibody, OSRC antibody, p104 antibody, P105-RB antibody, pp105 antibody, PP110 antibody, pRb antibody, Rb antibody, Rb-1 antibody, RB11 antibody
Specificity Retinoblastoma antibody detects endogenous levels of Retinoblastoma only when phosphorylated at threonine 826
Cross Reactivity Mouse, Rat
Applications ELISA, IF, WB
Immunogen Retinoblastoma antibody was raised in Rabbit using synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of threonine 826 (K-M-TP-P-R) as the immunogen

Images

Western Blot analysis using Retinoblastoma antibody (Thr826) (70R-33655)

Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using Retinoblastoma (Phospho-Thr826) antibody.

Specifications

Host Rabbit
Isotype IgG
Residues Human: Thr826, Mouse: Thr817, Rat: Thr797
Form & Buffer Supplied in PBS buffer (without Mg2+and Ca2+), pH 7.4, 150mM NaCl with 0.02% sodium azide and 50% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:1000

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles

General Information

Biological Significance Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate Retinoblastoma protein, this can lead to cancer.

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Images

  • Western Blot analysis using Retinoblastoma antibody (Thr826) (70R-33655) | Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using Retinoblastoma (Phospho-Thr826) antibody.
  • Immunofluorescent staining using Retinoblastoma antibody (Thr826) (70R-33655) | Immunofluorescence analysis of COS7 cells, using Retinoblastoma (Phospho-Thr826) antibody.

Availability: In stock

Price: €230.67
Size: 100 ug
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