RFP Tag antibody (10R-2938)
Mouse monoclonal RFP Tag antibody
Overview
Overview
| Synonyms | Monoclonal RFP Tag antibody, anti-RFP Tag antibody, Anti RFP Tag antibody, RFP antibody, RFP antibody, RFP Tag antibody, RF5R antibody, RF5R antibody, Red Fluorescent Protein antibody, Red Fluorescent Protein antibody |
|---|---|
| Specificity | RFP |
| Applications | Dot Blot, ELISA, IP, WB |
| Immunogen | RFP Tag antibody was raised in mouse using RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates as the immunogen. |
Images
Western Blot analysis using RFP Tag antibody (10R-2938)
1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with RFP vector; unstransfected control (1), transfected with TurboRFP (2) and transfected with dsRed (3)
Specifications
| Host | Mouse |
|---|---|
| Clone | RF5R |
| Isotype | IgG1 |
| Method of Purification | RFP Tag antibody was purified by Protein A affinity chromatography. |
| Form & Buffer | Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3 |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:1000-3000 |
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Storage & Safety
| Storage | Store at 4 deg C for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Red Fluorescent Protein is a protein which exhibits bright red fluorescence when exposed to blue light. Fluorescent proteins are members of a structurally homologous class of proteins that share the unique property of being self-sufficient to form a visible wavelength chromophore from a sequence of 3 amino acids within their own polypeptide sequence. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy. |
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