RFP Tag antibody (10R-2938)

Mouse monoclonal RFP Tag antibody

Synonyms Monoclonal RFP Tag antibody, anti-RFP Tag antibody, Anti RFP Tag antibody, RFP antibody, RFP antibody, RFP Tag antibody, RF5R antibody, RF5R antibody, Red Fluorescent Protein antibody, Red Fluorescent Protein antibody
Specificity RFP
Applications Dot Blot, ELISA, IP, WB
Immunogen RFP Tag antibody was raised in mouse using RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates as the immunogen.

Images

Western Blot analysis using RFP Tag antibody (10R-2938)

1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with RFP vector; unstransfected control (1), transfected with TurboRFP (2) and transfected with dsRed (3)

Specifications

Host Mouse
Clone RF5R
Isotype IgG1
Method of Purification RFP Tag antibody was purified by Protein A affinity chromatography.
Form & Buffer Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1000-3000

Storage & Safety

Storage Store at 4 deg C for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Red Fluorescent Protein is a protein which exhibits bright red fluorescence when exposed to blue light. Fluorescent proteins are members of a structurally homologous class of proteins that share the unique property of being self-sufficient to form a visible wavelength chromophore from a sequence of 3 amino acids within their own polypeptide sequence. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.

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Images

  • Western Blot analysis using RFP Tag antibody (10R-2938) | 1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with RFP vector; unstransfected control (1), transfected with TurboRFP (2) and transfected with dsRed (3)

Availability: In stock

Price: €106.34
Size: 100 ug
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