RhoC protein (His tag) (80R-1400)
Purified recombinant Human RhoC protein
|Synonyms||RHOH9 protein, ARH9 protein, H9 protein, ARHC protein, Rho-related GTP-binding protein RhoC|
Coomassie Blue stained SDS-PAGE of RhoC protein (His tag) (80R-1400)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-190 amino acids: MGSSHHHHHH SSGLVPRGSH MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYIADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS AKTKEGVREV FEMATRAGLQ VRKNKRRRGC|
|Grade & Purity||> 90% pure|
|Molecular Weight||23.8 (210aa) confirmed by MALDI-TOF (Real molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RhoC, also known as rho-related GTP-binding protein RhoC, is a small signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. RhoC promotes reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Recombinant human RhoC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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