Rhodamine antibody (10C-CR2050M1)
Mouse monoclonal Rhodamine antibody
Overview
Overview
| Synonyms | Monoclonal Rhodamine antibody, Anti-Rhodamine antibody, Rhodamine antibody, TRITC antibody, Anti Rhodamine antibody, TRITC antibody |
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| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Rhodamine antibody was raised in Mouse using This protein A purified monoclonal antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH. as the immunogen. |
Images
Rhodamine core structure
TRITC (tetramethylrhodamine-5-(and-6)-isothiocyanate) is the base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group (–N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules.
Specifications
| Host | Mouse |
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| Clone | 11H10 |
| Method of Purification | Rhodamine antibody was purified by Protein A affinity chromatography. |
| Form & Buffer | 0.02M K3PO4, pH 7.2, with 0.15M NaCl and 0.01% NaN3. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:50,000, IHC: 1:1,000-1:10,000, WB: 1:2,000-1:20,000 |
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Storage & Safety
| Storage | Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. This product is stable for several weeks at 4 deg C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Rhodamine is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as a dye and as a dye laser gain medium. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. |
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