rnhA protein (His tag) (80R-1934)
Purified recombinant rnhA protein
|Synonyms||Ribonuclease HI protein, sin protein, rnh protein, dasF protein, degrades RNA of DNA-RNA hybrids. protein, herA protein, JW0204 protein, cer protein, sdrA protein, ECK0214 protein|
|Assay Information||rnhA protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.|
Coomassie Blue stained SDS-PAGE of rnhA protein (His tag) (80R-1934)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-155 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV|
|Grade & Purity||> 95% pure|
|Molecular Weight||20 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 10% glycerol and 2mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant E. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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