RNP-Sm protein (30-AR71)
Purified native Bovine RNP-Sm protein
Overview
Overview
| Species | Bovine |
|---|---|
| Protein Type | Native |
| Applications | ELISA |
Specifications
| Source | Calf Thymus |
|---|---|
| Grade & Purity | > 90% pure |
| Molecular Weight | 30-35 kDa, 15 kDa |
| Form & Buffer | 20mM Hepes-NaOH, pH 7.5, with 0.32M KCl, 10mM MgCl2, 0.2mM dithiothreitol, 0.1mM PMSF, 20% glycerol, and 0.1% Mirc-O-protect. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | 0.5-1 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Today the best investigated immunoreactive antigens are double-stranded DNA (dsDNA), single stranded DNA (ssDNA), Sm (Smith), sn-RNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonuleic acid as well as SS-A (Ro) and SS-B (La). The antigen Scl 70, a 70 kD molecular weight protein, also known as DNA-topoisomerase-1, is associated with scleroderma. |
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