Flow Cytometric assay using RPA1 antibody (10R-1208)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RPA1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
RPA1 antibody
Flow Cytometric assay using RPA1 antibody (10R-1208)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RPA1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
Western Blot analysis of immunized recombinant protein using RPA1 antibody (10R-1208)
Specifications
Host
Mouse
Clone
RPA15I373
Isotype
IgG1
Method of Purification
RPA1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma
cultured in a medium containing bovine IgG-depleted
(approximately 95%) fetal bovine serum.
Form & Buffer
RPA1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information
This antibody solution contains sodium azide (NaN )
as a preservative. There is a potential hazard that
NaN reacts with copper or lead to produce an explosive
compound. For safe disposal, the vial has to
be washed thoroughly with water. It is strongly adv
General Information
Biological Significance
Replication protein A1 functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange