Immunohistochemical staining using RPA14 antibody (70R-12487)
Immunohistochemical staining of paraffin-embedded lung CA patient tumor using RPA14 antibody at a dilution of 1:100
RPA14 antibody
Immunohistochemical staining using RPA14 antibody (70R-12487)
Immunohistochemical staining of paraffin-embedded lung CA patient tumor using RPA14 antibody at a dilution of 1:100
Western blot analysis using RPA14 antibody (70R-12487)
Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 15 % SDS PAGE gel and RPA14 antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
RPA14 antibody was purified by antigen-affinity chromatography
Molecular Weight
14 kDa
Form & Buffer
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.