RRAS protein (His tag) (80R-1299)
Purified recombinant Human RRAS protein
Overview
Overview
| Synonyms | Ras like protein TC21 protein, TC 21 protein, Oncogene TC21 protein, TC21 protein, Ras-related protein R-Ras C86394 protein, Oncogene RRAS2 protein, Related RAS viral (r ras) oncogene homolog 2 protein, Related RAS viral oncogene homolog 2 protein, Teratocarcinoma oncogene TC21., Ras related protein R Ras2 protein, Teratocarcinoma oncogene protein, RRAS 2 protein, protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of RRAS protein (His tag) (80R-1299)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-215 amino acids: MGSSHHHHHH SSGLVPRGSH MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPC |
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| Expression System | E.coli |
| Grade & Purity | > 85% pure |
| Molecular Weight | 25.3 kDa (235aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl. |
| Concentration | 0.5 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | RRAS, also known as ras-related protein R-Ras, belongs to the Ras family which functions in signal transduction pathways. This protein has been shown to interact with RASSF5, NCK1, Bcl-2, ARAF and RALGDS. RRAS can bind GTP and GDP, and it has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant RRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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