S100 Beta antibody (10-2318)
Recombinant Fab monoclonal S100 Beta antibody
Overview
Overview
| Synonyms | Monoclonal S100 Beta antibody, Anti-S100 Beta antibody, NEF antibody, Protein S100-B antibody, S-100 calcium-binding protein beta chain antibody, S-100 protein beta chain antibody, S-100 protein subunit beta antibody, S100 antibody, S100 beta antibody, S100 calcium binding protein B antibody, S100 calcium binding protein beta antibody, S100 calcium-binding protein B antibody, S100 calcium-binding protein beta antibody, S100B antibody, S100beta antibody |
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| Specificity | Human |
| Protein Type | Recombinant |
| Applications | ELISA |
| Assay Information | S100 Beta antibody pair: 10-2319 can be used as the capture antibody with 10-2318 as the detection antibody for detection of human S100 Beta in ELISA |
Specifications
| Host | Mouse |
|---|---|
| Clone | M72101 |
| Isotype | IgG1 kappa - Fab fragment |
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Method of Purification | S100 Beta antibodies screened using Phage Display system. Expressed in and purified from E. coli. |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in 50 mM Potassium Phosphate, 10 mM Boric Acid, 150 mM NaCl, pH 7 |
| Concentration | > 1 mg/ml |
Storage & Safety
| Storage | Store at -20 deg C in small aliquots. Avoid repeated freeze thawing. |
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General Information
| Biological Significance | S100 Beta weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. S100 Beta antibody was produced as a recombinant Fab monoclonal antibody in a Phage Display system. This anti-S100 Beta antibody is used for detection of human S100 Beta in ELISA. The concentration of the antibody ranges from 1 mg/ml to 25 mg/ml depending on the lot. Recombinant Fab monoclonal antibodies have a number of advantages over standard antibodies including; improved scale up for batch consistency, more efficient binding so less antibody required, more accurate binding meaning fewer conjugation issues, less non specific due to removal of the Fc region, more flexibility for purification and labeling through a C-terminal His tag. |
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S100 Beta antibody
Recombinant Fab monoclonal S100 Beta antibody