S100A3 protein (His tag) (80R-1834)
Purified recombinant S100A3 protein
|Synonyms||S100E protein, SA3 100, S100 calcium binding protein A3 protein, SA3 100 protein, S100A3, SA3-100 protein, SA3-100|
Coomassie Blue stained SDS-PAGE of S100A3 protein (His tag) (80R-1834)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-101 amino acids: MGSSHHHHHH SSGLVPRGSH MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q|
|Grade & Purity||> 90% pure|
|Molecular Weight||13.9 kDa (121aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 2mM DTT and 0.2M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||S100A3 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A3 binds calcium with low affinity and zinc with high affinity and is believed to play a role in the calcium-dependent differentiation of cuticle cells and in the formation of hair shafts. Recombinant human S100A3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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