S100B protein (His tag) (80R-1390)
Purified recombinant Human S100B protein
|Synonyms||S100 calcium binding protein beta chain protein, Protein S100-B protein, NEF protein, S100B, SB-100 protein, S100B, S100 calcium binding protein beta (neural) protein, S100beta protein, S100 protein, S100 protein, SB-100, SB 100, S100 calcium binding protein B protein, S100 protein beta chain protein, S 100 protein beta subunit protein, S 100 protein beta chain protein, SB 100 protein|
Coomassie Blue stained SDS-PAGE of S100B protein (His tag) (80R-1390)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-92 amino acids: MGSSHHHHHH SSGLVPRGSH MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE|
|Grade & Purity||> 90% pure|
|Molecular Weight||12.8 kDa (112aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Recombinant human S100B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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