S100P protein (His tag) (80R-1329)
Purified recombinant Human S100P protein
|Synonyms||Migration inducing gene 9 protein, SP 100 protein, SP-100 protein, Protein S100P protein, SP 100, S100 calcium binding protein P MIG9 protein, S100E protein, SP-100, S100P, S100 calcium binding protein P protein, S100 P.|
Coomassie Blue stained SDS-PAGE of S100P protein (His tag) (80R-1329)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-95 amino acids: MGSSHHHHHH SSGLVPRGSH MTELETAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD KDAVDKLLKD LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK|
|Grade & Purity||> 90% pure|
|Molecular Weight||12.6 kDa (115aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 1mM DTT and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||S100P is a Ca2+ binding protein that belongs to S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100P is involved in diverse biological functions but the exact role or mechanism of its action is still largely unknown. Upon binding of calcium ions S100P undergoes a conformational change that results in an exposure of a hydrophobic surface which allows the interaction with specific target proteins. Recombinant human S100P protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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