SAE1 antibody (70R-13557)

Affinity purified Rabbit polyclonal SAE1 antibody

Synonyms Polyclonal SAE1 antibody, Anti-SAE1 antibody, AOS1 antibody, SUA1 antibody, SUMO-activating enzyme subunit 1 antibody, UBLE1A antibody, SAE1, FLJ3091 antibody, activator of SUMO1 antibody, SAE 1, ubiquitin-like protein SUMO-1 activating enzyme antibody, SAE-1, SAE-1 antibody, HSPC140 antibody, SAE1 antibody, sentrin/SUMO-activating protein AOS1 antibody, SAE 1 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB

Images

Western blot analysis using SAE1 antibody (70R-13557)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and SAE1 antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification SAE1 antibody was purified by antigen-affinity chromatography
Molecular Weight 38 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.72 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using SAE1 antibody (70R-13557)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and SAE1 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using SAE1 antibody (70R-13557)</font></div>     | Immunohistochemical staining of paraffin-embedded A549 Xenograft using SAE1 antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using SAE1 antibody (70R-13557)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10 % SDS PAGE gel and SAE1 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using SAE1 antibody (70R-13557)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAE1 antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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