SAE1 antibody (70R-50768)

Purified Polyclonal SAE1 antibody

Synonyms Polyclonal SAE1 antibody, Anti-SAE1 antibody, AOS1 antibody, SUA1 antibody, UBLE1A antibody, SUMO-activating enzyme subunit 1 antibody, Ubiquitin-like 1-activating enzyme E1A antibody
Specificity Recognizes endogenous levels of SAE1 protein
Cross Reactivity Monkey
Applications ICC, IF, IHC, WB
Immunogen SAE1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SAE1 as the immunogen

Images

Western blot analysis using SAE1 antibody (70R-50768)

Western blot analysis of SAE1 expression in Jurkat (A); A431 (B); HeLa (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification SAE1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance SAE1 mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

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Images

  • 

Western blot analysis using SAE1 antibody (70R-50768)



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Western blot analysis of SAE1 expression in Jurkat (A); A431 (B); HeLa (C) whole cell lysates.
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Immunohistochemical analysis using SAE1 antibody (70R-50768)



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Immunohistochemical analysis of SAE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using SAE1 antibody (70R-50768)



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Immunofluorescent analysis of SAE1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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