SART1 antibody (70R-12724)

Affinity purified Rabbit polyclonal SART1 antibody

Synonyms Polyclonal SART1 antibody, Anti-SART1 antibody, squamous cell carcinoma antigen recognised by T cells antibody, SART-1 antibody, MGC2038 antibody, Ara1 antibody, SART 1 antibody, SART1, SART 1, HOMS1 antibody, SART-1, SART1259 antibody, SART1 antibody, hSnu66 antibody, SNRNP110 antibody, Snu66 antibody, U4/U6.U5 tri-snRNP-associated protein 1 antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen SART1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 137 and 447 of SART1

Images

Western blot analysis using SART1 antibody (70R-12724)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1) using a 7.5 % SDS PAGE gel and SART1 antibody at a dilution of 1:5000

Specifications

Host Rabbit
Isotype IgG
Method of Purification SART1 antibody was purified by antigen-affinity chromatography
Molecular Weight 90 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.76 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:5000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using SART1 antibody (70R-12724)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1) using a 7.5 % SDS PAGE gel and SART1 antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunohistochemical staining using SART1 antibody (70R-12724)</font></div>     | Immunohistochemical staining of paraffin-embedded Cal27 xenograft using SART1 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using SART1 antibody (70R-12724)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: K562; D: NCI-H929) using a 7.5 % SDS PAGE gel and SART1 antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunofluorescent staining using SART1 antibody (70R-12724)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using SART1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Availability: In stock

Price: €323.11
Size: 100 ul
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